Figure 1 | Scientific Reports

Figure 1

From: Visualization of in vivo metabolic flows reveals accelerated utilization of glucose and lactate in penumbra of ischemic heart

Figure 1The alternative text for this image may have been generated using AI.

Focused microwave irradiation is the most suitable tissue fixation method.

Hearts were prepared by three different methods: focused microwave irradiation (FMW), rapid freezing method, in which hearts were isolated immediately after thoracotomy and then quick-frozen in liquid N2, and by delayed freezing, whereby hearts were isolated 10 min after cervical dislocation. The time required for inactivation of postmortem enzymatic activity was shortest in FMW (0.96 s), followed by rapid freezing (approx. 20 s after thoracotomy) and delayed freezing (10 min after cervical dislocation). (a–c) Amounts of metabolites in the glycolytic pathway(a), tricarboxylic acid (TCA) cycle (b), values for NADH/NAD+ ratio (c) and amino acids (d) were determined by CE-MS. *P < 0.05, **P < 0.01 (n = 6).

Back to article page