Figure 2 | Scientific Reports

Figure 2

From: Visualization of in vivo metabolic flows reveals accelerated utilization of glucose and lactate in penumbra of ischemic heart

Figure 2The alternative text for this image may have been generated using AI.

Quantitative imaging of cardiac metabolites in ischemic hearts.

(a) Representative images of NADH, lactate, succinate, lactate/pyruvate ratio, ATP, ADP, AMP are shown. The red region in the optimal image indicates the area with increased NADH as a metabolic indicator for the ischemic region. We repeated the experiments three times with reproducible results (see Supplementary Fig. S10). (b) Mapping of energy charge (EC) values based on MALDI-IMS, normalized using capillary electrophoresis (CE)-MS-based quantitative analysis. Representative images of sham-operated (sham) or 10 min post–myocardial infarction (MI) mice are shown. Experiments were repeated three times with reproducible results (see Supplementary Fig. S10). (c) The EC map was subdivided into three sectors: a region of ischemia (ischemic), a region adjacent to ischemia (penumbra) and a region distant to ischemia (normoxic). Values for EC in these sectors are shown as histograms. Sham, a heart obtained from a sham-operated mouse. (d) Elevated production of adenosine was detected in correlation with ischemic severity (left), which overflowed into the penumbra region from the ischemic core (arrow in right panel).

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