Figure 6
From: The porin VDAC2 is the mitochondrial platform for Bax retrotranslocation
Bax specifically binds VDAC2 on the OMM.
(A) Distribution of endogenous Bax, Bcl-xL and VDAC between mitochondria-containing heavy membrane (mitochondria, right) fraction, ER-containing light membrane (center) and cytosol (left) in wild-type (WT), Bax/Bak DKO, VDAC2 KO (2KO) and VDAC1/3 DKO (1/3DKO) MEFs. Tom20 and AKT1 serve as loading controls. Fractionation is controlled by corresponding wild-type MEF fractions (HM: heavy membrane, LM: light membrane, C: cytosol). n = 3. (B) Western blot analysis of endogenous Bax and Bcl-xL levels in wild-type, Bax/Bak DKO, VDAC2 KO and VDAC1/3 DKO MEFs. Tom20 serves as loading control. n = 3. (C) Bax (blue) translocation to the mitochondria targets VDAC2 (purple) in the outer mitochondrial membrane (OMM) and VDAC2 serves as platform for Bax retrotranslocation.
