Figure 5 | Scientific Reports

Figure 5

From: MiR 3180-5p promotes proliferation in human bladder smooth muscle cell by targeting PODN under hydrodynamic pressure

Figure 5

The miRNA 3180-5p regulates the expression of PODN.

After upregulation of miR 3180-5p by transfection, (a) the mRNA level of ADARB1was increased (p = 0.008), and (b) the mRNA level of PODN was markedly decreased (p = 0.001). (c) Adenosine deaminase RNA-specific B1, the functional protein of ADARB1, had no change (p = 0.134). (d) The protein level of PODN was markedly decreased in the miR 3180-5p mimics-treated cells. After downregulation of miR 3180-5p via transfection, (e) the mRNA level of PODN was markedly increased (p = 0.0035), and (f) the protein level of PODN was markedly increased (p = 0.025). (g,h) T293T cells were co-transfected by psiCHECK-PODN (wild or mutant type) with mimics miR. The results showthat upon transfection with wild-type psiCHECK-PODN reporter vectors, luciferase activity was decreased by ~41% (p = 0.0022); moreover, mutation of the binding site markedly suppressed the effect of miR 3180-5p (p = 0.8127).

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