Figure 2
From: Synthesis and mechanisms of action of novel harmine derivatives as potential antitumor agents
Inhibitory role of compound 3c in cancer cells.
(A) Three human colorectal cancer cell lines, DLD1, HCT116 and RKO, were seeded in 96 well plates and treated with the indicated concentration of compound 3c for 24 h (left panel) or with the indicated time at the concentration of 8 μM (right panel). Cell viability was determined by CCK8 assays. (B) The number of colonies was counted after the treatment of HCT116 cells with the indicated concentration of compound 3c for 24 h. (n = 3, *P < 0.05 versus DMSO control). (C) Microphotographs of HCT116 cells treated with drug-free media (DMSO) or media containing 5 μM and 10 μM of compound 3c for 24 h. (D,E) Colony formation (left panel) and CCK8 (right panel) assays to determine drug effect on cancer and normal cells, HepG2/LO2 cells in D and HCT116/HIEC cells in E, respectively. Cells seeded in 6 well plates for colony formation assay or 96 well plates for CCK8 assay were treated with compound 3c at indicated concentration. For colony formation assay, n = 3, *P < 0.05 versus normal cells under same condition. For CCK8 assay, n = 6, *P < 0.05 versus normal cells under same condition. The data represents the means ± SDs.
