Figure 3
From: In vivo quantification of the secretion rates of the hemolysin A Type I secretion system
Detection of the surface exposed HlyA fragment of eGFP-HlyA by confocal laser scanning microscopy.
E. coli cells expressing eGFP-HlyA, HlyD, HlyB or HlyB-H662A in different combinations of the proteins. Shown is the eGFP fluorescence (left panel) of the fusion proteins, the HlyA mediated Cy3 fluorescence at the cell surface (second left panel), merged images of eGFP and Cy3 fluorescence (second right panel) and differential interference contrast (DIC) images of the cells (right panel). The different combinations of proteins employed are indicated to the left.
