Figure 6 | Scientific Reports

Figure 6

From: siRNA capsulated brain-targeted nanoparticles specifically knock down OATP2B1 in mice: a mechanism for acute morphine tolerance suppression

Figure 6

siRNA (OATP2B1) capsulated NPs function performance in acute morphine tolerance mice.

OATP2B1 expression analyzed in mice (A) cerebrums or (B) epencephalons which treated with DGL-PEG/dermorphin or DGL-PEG/dermorphin/siRNA (OATP2B1) NPs Na2SO4 DEPC solutions for 48 h before morphine induced acute tolerance generates; (C) OATP2B1 (brown) detected by immunohistochemistry in brain coronal section of mouse; (D) The maximal possible effect (MPE%) was calculated from the data of trail-flick assay in each group of saline or morphine treated mice after acquiring with DGL-PEG/dermorphin or DGL-PEG/dermorphin/siRNA (OATP2B1) NPs Na2SO4 DEPC solutions for 48 h. Pairwise comparisons were calculated by student t-test to calculate P values (*P < 0.05, ***P < 0.001) (n = 6).

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