Figure 2 | Scientific Reports

Figure 2

From: Autophagy-related proteins are functionally active in human spermatozoa and may be involved in the regulation of cell survival and motility

Figure 2

LC3-II/LC3-I ratio in basal conditions.

Fresh semen was washed and proteins were extracted and further resolved in SDS-PAGE (see material and methods section). Immunoblotting was performed with an antibody that recognized both LC3-I and LC3-II proteins. Immunoblotting is a representative image of the protein expression of LC3 from 8 semen donors (lanes numbered from 1 to 8) from a total of 33. LC3-I and LC3-II were measured and autophagy flux was expressed as ratio of LC3-II/LC3-I.

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