Table 2 Effect of chloroquine and rapamycin in human sperm velocity parameters.

From: Autophagy-related proteins are functionally active in human spermatozoa and may be involved in the regulation of cell survival and motility

Velocity Parameters

Control

Chloroquine (50 μM)

Rapamycin (500 nM)

VCL (μm/s)

64.24 ± 4.54

60.90 ± 6.28

66.52 ± 5.44

VSL (μm/s)

25.35 ± 2.09

18.53 ± 1.90*

28.25 ± 1.15

VAP (μm/s)

36.52 ± 2.30

31.88 ± 2.78

38.84 ± 2.60

LIN (%)

39.76 ± 2.18

30.60 ± 1.56

43.28 ± 3.10

STR (%)

69.37 ± 2.44

57.95 ± 2.86*

73.36 ± 3.10

WOB (%)

57.18 ± 1.37

52.78 ± 1.33*

58.78 ± 1.99

ALH (μm)

3.06 ± 0.13

3.09 ± 0.47

2.85 ± 0.08

BCF (Hz)

9.34 ± 0.62

6.49 ± 1.36

9.44 ± 0.58

  1. Velocity parameters were assessed by CASA system after 2 hours of incubation with chloroquine (50 μM) or rapamycin (500 nM). VCL: Curvilinear velocity. VSL: Linear velocity. VAP: Mean velocity. LIN: Linearity coefficient. STR: Straightness coefficient. WOB: Wobble coefficient. ALH: Mean lateral head displacement. BCF: Frequency of head displacement. Values represent the mean + SEM of four separate experiments (*p < 0.05 compared with untreated control containing only vehicle).
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