Table 1 Data of sRNA sequences in the NT, MET and AET library.

From: Identification of miRNAs and their targets in wild tomato at moderately and acutely elevated temperatures by high-throughput sequencing and degradome analysis

Types

NT library A

MET library A

AET library A

Total sRNA number

Percent of total (%)

Unique sRNA number

Percent of unique (%)

Total sRNA number

Percent of total (%)

Unique sRNA number

Percent of unique (%)

Total sRNA number

Percent of total (%)

Unique sRNA number

Percent of unique (%)

Raw reads

10,850,996

100.00

1,937,422

100.00

10,806,944

100.00

1,967,034

100.00

10,989,848

100.00

1,215,890

100

3ADT&length filter B

3,985,067

36.73

878,490

45.34

3,358,332

31.08

618,514

31.44

2,421,294

22.03

513,294

42.22

Junk reads

21,863

0.20

10,187

0.53

28,840

0.27

14,522

0.74

30,510

0.28

6,570

0.54

Rfam C

720,517

6.64

50,539

2.61

580,535

5.37

40,566

2.06

675,042

6.14

34,628

2.85

mRNA

2,989,464

27.55

183,508

9.47

3,031,582

28.05

210,920

10.72

3,564,171

32.43

112,495

9.25

Repeats D

9,757

0.09

774

0.04

9,021

0.08

827

0.04

5,887

0.05

476

0.04

rRNA

568,456

5.24

34,268

0.32

444,594

4.11

26,649

0.25

514,197

4.68

23,682

0.22

tRNA

111,052

1.02

9,357

0.09

104,212

0.96

8,066

0.07

128,180

1.17

6,867

0.06

snoRNA

3,106

0.03

925

0.01

2,952

0.03

853

0.01

2,748

0.03

547

0

snRNA

2,354

0.02

1,237

0.01

2,680

0.02

1,213

0.01

3,181

0.03

741

0.01

other Rfam RNA

35,549

0.33

4,752

0.04

26,097

0.24

3,785

0.04

26,736

0.24

2,791

0.03

Valid reads

3,333,687

30.72

829,756

42.83

3,964,329

36.68

1,095,508

55.69

4,483,258

40.79

560,147

46.07

  1. ANT, control at normal temperature; MET, heat stress at moderately elevated temperature; AET, heat stress at acutely elevated temperature.
  2. BReads lacking three ADTs or with lengths <19 nt or >24 nt were removed.
  3. CCollection of many common noncoding RNA families other than micro RNAs; http://rfam.janelia.org.
  4. DDownloaded from http://www.girinst.org/repbase.