Figure 1: Schematic design of 3D culture device providing mechanical stress and air-exposure to cells. | Scientific Reports

Figure 1: Schematic design of 3D culture device providing mechanical stress and air-exposure to cells.

From: Co-stimulation of HaCaT keratinization with mechanical stress and air-exposure using a novel 3D culture device

Figure 1

(a) An all-in one device was fabricated using PDMS. The device fits into 6-well plates and has 11 holes around the wall to diffuse media between reservoir and upper chamber. (b) Cultivation process of 3D skin under mechanical stretch and air-exposure. For differentiating HaCaT, culture medium was removed out of reservoir. The 3D skin was cultured with intermittent stretch for 14 days by injecting water in the lower chamber. (c) Collagen matrix without nanofiber was shrunk in the media in 24 h. (d) Collagen matrix with nanofiber was fixed by nanofiber in place after 24 h.

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