Figure 2: Morphological comparison of the regenerated epidermis under co-stimulation with mechanical stress and air-exposure. | Scientific Reports

Figure 2: Morphological comparison of the regenerated epidermis under co-stimulation with mechanical stress and air-exposure.

From: Co-stimulation of HaCaT keratinization with mechanical stress and air-exposure using a novel 3D culture device

Figure 2

SEM images showing epidermis morphology of 3D skin cultured under non-stretch or intermittent-stretch conditions. (a,b) Collagen fiber (arrow) was covered with smooth epidermis of HaCaT under air-exposure only condition. (d,e) Collagen fiber was covered with tough stratum corneum of HaCaT differentiated under co-stimulation with intermittent stretch and air-exposure. (c,f) Epidermal layer was labeled using FITC-conjugated nanoparticles. (c) Nanoparticles were deposited into the epidermal layer but distinguishable granular layer was not detected. (f) Spinous or granular layers were confirmed, stained with FITC-labeled nanoparticles. Inside figure is the rat skin which was deposited with FITC-labeled nanoparticles. But, Nanoparticles also were not penetrated to the dermal layer of rat skin. (Bar = 10 μm (a,d), 100 μm (b,e) and 50 μm (c,f)).

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