Figure 3: Confluency and qRT-PCR analysis of keratinized 3D skins. | Scientific Reports

Figure 3: Confluency and qRT-PCR analysis of keratinized 3D skins.

From: Co-stimulation of HaCaT keratinization with mechanical stress and air-exposure using a novel 3D culture device

Figure 3

Nucleated cell number was compared between NS and IS (a). mRNA levels of differentiation markers were quantified at 7 days (b) and 14 days (c). (a) Nucleated cell number on day 14 in IS decreased than NS. (b) Differentiation markers did not show significant differences between NS and IS. (c) Expression of K1 was lower but those of Inv and Lor were higher in IS. These findings indicate that cells were keratinized following transition process from basal layer to granular layer. Further, the increased Lor implies that HaCaT reached terminal differentiation. Fil did not show significant differences (*p < 0.05, **p < 0.01, ***p < 0.001).

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