Figure 4: Comparison of expressions of differentiation markers and formation of cornified envelops of 14-day cultured 3D skins between non-stretch and intermittent stretch conditions. | Scientific Reports

Figure 4: Comparison of expressions of differentiation markers and formation of cornified envelops of 14-day cultured 3D skins between non-stretch and intermittent stretch conditions.

From: Co-stimulation of HaCaT keratinization with mechanical stress and air-exposure using a novel 3D culture device

Figure 4

Filaggrin (a,d) and loricrin (b,e) were immunostained in red and the nucleus was stained in blue. Cornified envelops (c,f) were identified by Nile red staining of lipids. (a,b) Both filaggrin and loricrin were less expressed under air-exposure without stretch. (d,e) Both filaggrin and loricrin were highly expressed under air-exposure with intermittent stretch. (c) Cornified envelops were rarely detected under air-exposure only condition. (f) High density of cornified envelops was detected with high lipid contents under co-stimulation with intermittent stretch and air-exposure. Bar = 50 μm.

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