Figure 1: Protein extraction from formalin-fixed cells is comparable to fresh cells when lysing protocol includes high concentrations of Tris-HCl, anionic detergent SDS, and high heat.
From: Protein characterization of intracellular target-sorted, formalin-fixed cell subpopulations
(a) Expression of TUBB3 (green) in cell lines SH-SY5Y and SK-N-MC. Scale bar, 200 μm. DAPI, 4′,6-diamino-2-phenylindole (blue). (b) Total protein isolated from formalin-fixed cells was compared to fresh cells under two different lysing conditions (4 °C, ice; 100 °C, boil). Data represented as mean ± standard deviation (s.d.) from four, independent experiments. Within cell lines, one-way ANOVAs were performed to determine significance (unlike letters are significantly different from each other, P < 0.02). (c) Protein quality assessed by Coomassie Blue stained PAGE gel for general banding and WB for POI.
