Figure 5: Methodology schematic.
From: Protein characterization of intracellular target-sorted, formalin-fixed cell subpopulations
(1) Isolate tissue of interest and dissociate cells; (2) Fix samples in suspension with 10% formalin; (3) Label cells with a specific intracellular marker for the cell population of interest and select for cells using FACS; (4) Extract protein from collected samples, and run protein assays. Ab, antibody.
