Figure 2: p38 MAPK and c-Jun are involved in the TGF-β-regulated expression of key cholesterol uptake genes in human macrophages. | Scientific Reports

Figure 2: p38 MAPK and c-Jun are involved in the TGF-β-regulated expression of key cholesterol uptake genes in human macrophages.

From: The role of mitogen-activated protein kinases and sterol receptor coactivator-1 in TGF-β-regulated expression of genes implicated in macrophage cholesterol uptake

Figure 2

Knockdown of GAPDH or p38 MAPK or c-Jun expression in THP-1 monocytes, differentiation of monocytes into macrophages and incubation with vehicle (−, empty bars) or TGF-β (30 ng/ml) (+, filled bars) for 24 h was carried out as in Fig. 1. Total RNA was subjected to RT-qPCR using primers against CD36, SR-B1, SR-A1, LPL or RPL13A. The mRNA expression levels were determined using the comparative Ct method and normalized to RPL13A with the value from vehicle treated cells arbitrarily assigned as 1. The data represent mean ± SD of three independent experiments. Statistical analysis was performed using the two-tailed unpaired Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001.

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