Figure 4: AP-1 does not interact with the TGF-β response element in the regulatory region of the LPL gene. | Scientific Reports

Figure 4: AP-1 does not interact with the TGF-β response element in the regulatory region of the LPL gene.

From: The role of mitogen-activated protein kinases and sterol receptor coactivator-1 in TGF-β-regulated expression of genes implicated in macrophage cholesterol uptake

Figure 4

EMSA were carried out using radiolabelled +9/+49 or +46/+90 sequence and whole cell extracts from THP-1 macrophages that were either untreated (0 h) or incubated with 30 ng/ml of TGF-β for 24 h (24 h). +Indicates competition with a 400-fold molar excess of unlabelled complementary oligonucleotide (self), Sp1/Sp3 binding site (Sp1/Sp3) or AP-1 binding site (AP-1). The DNA-protein interactions with the +9/+49 and the +46/+90 sequences have been previously characterized18,22. The region of the autoradiogram containing the Sp1/Sp3 DNA protein complexes (indicated by a vertical line) is shown. The data are representative of three independent experiments.

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