Figure 5: The TGF-β-mediated inhibition of minimal LPL promoter activity is attenuated by transfection of SRC-1 expression plasmid but not that for p300/CBP. | Scientific Reports

Figure 5: The TGF-β-mediated inhibition of minimal LPL promoter activity is attenuated by transfection of SRC-1 expression plasmid but not that for p300/CBP.

From: The role of mitogen-activated protein kinases and sterol receptor coactivator-1 in TGF-β-regulated expression of genes implicated in macrophage cholesterol uptake

Figure 5

U937 cells were transfected with the minimal LPL promoter-luciferase construct (−101/+187 in the pGL2 Basic-luciferase vector) and pcDNA3 control vector (pcDNA3) or p300/CBP expression plasmid (p300) (1.5 μg) (A) or SRC-1 expression plasmid (SRC-1) (1.5 and 3.0 μg as indicated) (B). The cells were then differentiated with PMA (1 μM) for 12 h and then either treated with vehicle (−, empty bars) or TGF-β (30 ng/ml) for further 12 h (+, filled bars). The luciferase activity was normalized to the protein concentration and is expressed as Relative Luciferase Activity with the value in cells transfected with the control pcDNA3 plasmid and treated with vehicle arbitrarily assigned as 100%. The data represent mean ± SD from three independent experiments. Statistical analysis was performed using one-way ANOVA with Tukey’s post-hoc test, *p < 0.05, **p < 0.01, ***p < 0.001.

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