Figure 7: Knockdown of SRC-1 affects LPL gene expression.
Knockdown of SRC-1 expression in THP-1 monocytes, differentiation of monocytes into macrophages and incubation with vehicle (−, empty bars) or TGF-β (30 ng/ml) (+, filled bars) for 24 h was carried out as in Fig. 1. (A) Equal amounts of protein extracts were subjected to western blot analysis using antisera against SRC-1 or β-actin. The image shows the signal from the immunoreactive SRC-1 (160 kDa) or β-actin (42 kDa). The histogram below the image shows protein expression of SRC-1 normalized to β-actin and is expressed as a fold change relative to GAPDH siRNA-transfected cells (arbitrarily assigned as 1). The data represent mean ± SD of three independent experiments. Statistical analysis was performed using the Student’s t-test, *p < 0.05. (B) RT-qPCR on total RNA was carried out using primers against LPL or RPL13A. The mRNA expression levels were determined using the comparative Ct method and normalized to RPL13A. The value in cells transfected with GAPDH siRNA and treated with vehicle was arbitrarily assigned as 1. The data represent mean ± SD of five independent experiments. Statistical analysis was performed using one-way ANOVA with Tukey’s post-hoc test, ***p < 0.001.
