Figure 1 | Scientific Reports

Figure 1

From: Cigarette smoke reversibly activates hypoxia-inducible factor 1 in a reactive oxygen species-dependent manner

Figure 1

Hypoxia-inducible factor 1α (HIF-1α) is induced by cigarette smoke extract (CSE) in vitro.

Human lung adenocarcinoma A549 cells (a), human bronchial epithelial BEAS-2B cells (b) and human cervical carcinoma HeLa cells (c) were exposed to the indicated concentrations of CSE in the presence of 20% O2, 100 μM desferrioxamine (DFX), or 1% O2 for 4 h. Whole-cell lysates were immunoblotted (IB) using anti-HIF-1α, HIF-1β and β-actin antibodies.

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