Figure 5
Impact of kinase inhibitors on cigarette smoke extract (CSE)-induced hypoxia-inducible factor 1 (HIF-1) activation.
A549 cells were exposed to 2% CSE for the indicated time-periods (a,b) prior to immunoblotting (IB) whole cell lysates for phosphorylated and total-p42/44 mitogen-activated protein kinase (MAPK) (a) or Akt (b) and β-actin. (c) A549 cells were incubated with or without 2% CSE for 4 h in the presence of 20-μM PD98059 (PD), 25-μM LY294002 (LY), or 100-μM SC-514 (SC) under 20% O2 prior to IB of whole cell lysates, as indicated. (d) A549 cells were exposed to 2% CSE with or without the NF-κB inhibitors, 20-μM BAY11-7082 (BAY) or and 20-μM resveratrol (RES) for 4 h prior to IB of whole cell lysates, as indicated.
