Figure 6
Involvement of reactive oxygen species (ROS) in hypoxia-inducible factor 1 (HIF-1) activation.
(a) ROS generation in A549 cells was monitored by loading with 2′,7′-dichlorodihydrofluorescin diacetate at 37 °C for 20 min prior to 2% CSE exposure for 4 h. A549 cells were incubated with or without 2% CSE and 10 mM N-acetylcysteine (NAC) under 20% O2 for 4 h prior to (b) whole-cell lysate immunoblotting (IB) for HIF-1α and (c) determination of the mRNA levels of vascular endothelial growth factor (VEGF), heme oxygenase-1 (HO-1) and regulated in development and DNA damage response 1 (REDD1) using real-time reverse transcriptase polymerase chain reaction. Data are presented as the mean ± SD; *p < 0.05, as compared with control (no treatment); #p < 0.05 for the indicated comparisons.
