Figure 4
From: l-Ornithine affects peripheral clock gene expression in mice
Effects of L-ornithine administration at ZT 1 on secretion of insulin, GLP-1, and glucose in plasma, and clock genes in the liver.
(a) Experimental schedule. Open and filled bars indicate light and dark periods, respectively. Mice were entrained to the light-dark cycle for at least 14 days. At ZT 1, l-ornithine (1,000 mg/kg) or vehicle was administered to mice after 24 h fasting by oral injection (filled arrowhead); at ZT 2, plasma and tissues were collected (open arrowhead). (b) RT-PCR analysis of clock genes in the liver. mRNA expression was normalized to P0. (c–e) Insulin, glucose and total length GLP-1 concentration in plasma (mean ± SEM, n = 10 mice respectively). **P < 0.01, *P < 0.05 versus control (Mann-Whitney U test).
