Figure 2 | Scientific Reports

Figure 2

From: A fragment-based approach applied to a highly flexible target: Insights and challenges towards the inhibition of HSP70 isoforms

Figure 2

SPR fragment screening hits.

(A) Chemical structure and HSC70-NBD SPR KD of 7. (B) Chemical structure and HSC70-NBD SPR KD of 8. (C) Concentration-response SPR experiment showing the binding of 7 to wild-type HSC70-NBD. (D) Concentration-response SPR experiment showing the binding of 8 to wild-type HSC70-NBD. (E) SPR binding curves for 7 binding to wild-type HSC70-NBD (light-blue) and to the S275W (purple) and S275F (blue) HSC70-NBD mutants. (F) SPR binding curves for 8 binding to wild-type HSC70-NBD (light-blue) and to the S275W (purple) and S275F (blue) HSC70-NBD mutants. (G) Structure of 7 (yellow) bound to the adenine sub-pocket in HSC70/BAG1. (H) Structure of 8 (yellow) bound to the adenine sub-pocket in HSC70/BAG1. In both panels the Fo-Fc electron density omit map is shown in blue and contoured at 3 σ.

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