Table 2 Yeast strains and plasmids used in this study.
Strain / Plasmid | Genotype and description | Reference |
|---|---|---|
S. cerevisiae strains | ||
 CEN.PK2-1C | MAT a ura3-52 trp1-289 leu2-3,112 his3Δ1 MAL2-8C SUC2 | EUROSCARF |
 JHY5101 | CEN.PK2-1C gsf2Δ::loxP-URA3-loxP | This study |
 JHY5102 | CEN.PK2-1C mig1Δ::loxP-URA3-loxP | This study |
 JHY5103 | CEN.PK2-1C hxk2Δ::loxP-his5+-loxP | This study |
 JHY5160 | CEN.PK2-1C dld1Δ::loxP jen1Δ::loxP adh1Δ::loxP gpd1Δ::loxP gpd2Δ::loxP | |
 JHY5210 | JHY5160 pdc1Δ::PTEF1-Lm.ldhA-TCYC1 | |
 JHY5212 | JHY5210 gsf2Δ::loxP-his5+-loxP | This study |
 JHY5213 | JHY5210 syn8Δ::loxP-his5+-loxP | This study |
 JHY5214 | JHY5210 stm1Δ::loxP-his5+-loxP | This study |
 JHY5215 | JHY5210 sif2Δ::loxP-his5+-loxP | This study |
 JHY5216 | JHY5210 mig1Δ::loxP-his5+-loxP | This study |
 JHY5217 | JHY5210 hxk2Δ::loxP-his5+-loxP | This study |
 JHY5310 | Evolved strain from JHY5210 | |
 JHY602 | CEN.PK2-1C adh1Δ::loxP adh2Δ::loxP adh3Δ::loxP adh4Δ::loxP adh5Δ::loxP | |
 JHY5401 | JHY602 gsf2Δ::loxP-URA3-loxP | This study |
 JHY604 | CEN.PK2-1C adh1Δ::loxP gpd1Δ::loxP gpd2Δ::loxP | |
 JHY5402 | JHY604 gsf2Δ::loxP-URA3-loxP | This study |
Plasmids | ||
 pUG27 | Plasmid containing loxP-his5+-loxP deletion cassette | EUROSCARF |
 pUG72 | Plasmid containing loxP-URA3-loxP deletion cassette | EUROSCARF |
 p416GPD | CEN/ARS plasmid, URA3, PTDH3, TCYC1 | |
 p416GPD-GSF2 | CEN/ARS plasmid, URA3, PTDH3-GSF2-TCYC1 | This study |
 p416GPD-SYN8 | CEN/ARS plasmid, URA3, PTDH3-SYN8-TCYC1 | This study |
 p416GPD-STM1 | CEN/ARS plasmid, URA3, PTDH3-STM1-TCYC1 | This study |
 p416GPD-SIF2 | CEN/ARS plasmid, URA3, PTDH3-SIF2-TCYC1 | This study |
