Figure 3

Suppression of CD200R inhibits pro-inflammation polarization of microglia and enhances microglial anti-inflammatory response.

BV2 cells were incubated with scrambled RNA or si_CD200R, followed by treatment IL-4 (10 ng/ml), LPS (100 ng/ml), or CD200 (500 ng/ml) for 3 h. Supernatants were collected and assayed for levels of TNF-α (A) and IL-10 (B) by ELISA. (C) mRNA fold changes for Arg1 were calculated for treatment with scr and si_CD200R under control conditions (Cont) and after treatment with CD200. After 3 h, total RNA was prepared from BV2 cells and used for quantitative real-time PCR. Relative mRNA expression levels of Arg1 are presented as fold induction. Data are representative of three experiments. ***P < 0.001 vs. control.