Figure 4

Foxp3 over-expression enhances Arg1 positive microglial activation.

(A) BV2 cells were transfected with mock (pcDNA) or Flag-tagged-Foxp3-GFP (Foxp3-GFP) plasmids for 24 h and Foxp3 expression was analysed by immunoblotting and immunocytochemistry with DAPI. (B) BV2 cells transfected with mock (pcDNA) or Foxp3 plasmids (Foxp3-GFP) were cultured with IL-4 (10 ng/ml) or CD200 (500 ng/ml) for 3 h. Total RNA was prepared from BV2 cells for analysis using quantitative real-time PCR. Relative mRNA expression levels of Arg1 are presented as fold induction. Data are representative of three experiments. ***P < 0.001 vs. control.