Table 2 Summary of statistics for production of transgenic marmosets.

From: Generation of transgenic marmosets expressing genetically encoded calcium indicators

Viral construct

Collection method

Embryos transferred

ET procedures

Confirmed pregnancies (%)

Spontaneous miscarriages

Successful deliveries

Live births (% birth rate)

Animal ID

CMV-GCaMP5g

NAT

16

6

3 (50)

1

2

2 (33.3)

TG-Y, TG-D2

IVF

57

16

5 (10)

5

0

0 (0)

 

Total

73

22

8 (36.4)

6

2

2 (9.1)

 

CMV-mKO-GCaMP6s

NAT

22

8

2 (25)

1

1

1 (13)

TG-E

IVF

6

1

0 (0)

0

0

0 (0)

 

Total

28

9

2 (30)

1

1

1 (11.1)

 

hSyn-mKO-GCaMP6s

NAT

30

11

3 (27)

3a

0

0 (0)

 

IVF

35

9

3 (33)

1

2

3 (33)

TG-S, TG-L, TG-D1

Total

65

20

6 (30)

4

2

3 (15)

 

hSyn-GCaMP5g

NAT

7

2

0 (0)

0

0

0 (0)

 

IVF

50

14

2 (14)

1

1

1 (7.1)

TG-D3

Total

57

16

2 (12.5)

1

1

1 (6.3)

 

CMV-GCaMP6s

NAT

13

4

1 (25)

1

0

0 (0)

 

IVF

11

4

2 (50)

1

1

1 (25)

TG-J

Total

24

8

3 (37.5)

2

1

1 (12.5)

 

hSyn-GCaMP6s

IVF

28

7

1b (14.29)

0

ND

ND

 

Total

275

82

22 (26.8)

14

7

8 (9.8)

 
  1. IVF: Oocytes collected via laparotomy; NAT: Naturally fertilized embryos; ET: embryo transfer; TG: transgenic animal; ND: not yet determined.
  2. aStillborn fetuses were spontaneously miscarried after 102d, 135d, and 134d of gestation. They were named M01, M02, and M03, respectively.
  3. bCurrently ongoing singleton pregnancy confirmed by ultrasound examination.
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