Figure 1 | Scientific Reports

Figure 1

From: Arabidopsis aldehyde dehydrogenase 10 family members confer salt tolerance through putrescine-derived 4-aminobutyrate (GABA) production

Figure 1The alternative text for this image may have been generated using AI.

In vitro production of GABA or β-alanine by recombinant AtALDH10A8 and AtALDH10A9.

For assay of ABAL-dependent activities, 50 nM protein was used with 0.1 mM NAD+ and 50 μM ABAL for AtALDH10A8, or 0.5 mM NAD+ and 800 μM ABAL for AtALDH10A9; for assay of APAL-dependent activities, the reactions contained 10 nM protein with 0.1 mM NAD+ and 20 μM APAL for AtALDH10A8 or 0.5 mM NAD+ and 50 μM APAL for AtALDH10A9. Data represent the mean of three technical replicates from a typical enzyme preparation. Closed and open circles indicate GABA and β-alanine concentration, respectively. The estimated rates of GABA production at 30 s were 0.59 and 8.9 μmol min−1 mg−1 protein for AtALDH10A8 and AtALDH10A9, respectively. The corresponding rates of β-alanine production were 3.3 and 4.8 μmol min−1 mg−1 protein, respectively. Substrate conversion after 15 min was 20–29% and 11–12%, respectively, for the ABAL- and APAL-dependent reactions. The assays were performed as 5-mL reactions.

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