Figure 2 | Scientific Reports

Figure 2

From: Arabidopsis aldehyde dehydrogenase 10 family members confer salt tolerance through putrescine-derived 4-aminobutyrate (GABA) production

Figure 2The alternative text for this image may have been generated using AI.

Subcellular localization of AtALDH10A8 and AtALDH10A9 fusion proteins in Arabidopsis cell suspension protoplasts.

Confocal laser scanning microscopic images of protoplasts transiently co-expressing GFP fusion protein and peroxisomal, cytosolic or plastid markers. (A) GFP-AtALDH10A8 (a,e) was co-expressed with a mCherry peroxisome marker (b) or RFP cytosol (f). AtALDH10A8-GFP (i) was co-expressed with RFP plastid marker (j). (B) GFP-AtALDH10A9 (m) and AtALDH10A9-GFP (q) were co-expressed with mCherry peroxisomal marker (n) or a RFP cytosol marker (r). Merged images of the green and red signals appear yellow (c,g,k,o,s). Cell morphology was observed with transmitted light microscopy (d,h,i,p,t). Images were taken 16 h after protoplast transformation. Scale bars = 10 μm.

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