Figure 6

Schematic diagram depicting three subcellular compartments for GABA synthesis in Arabidopsis.
Enzymes are shown in blue letters, with biochemically characterized enzymes indicated by bold lettering. Dashed arrows indicates enzymes for which subcellular localization has not been studied. In silico analysis predicts three putative CuAOs (At1g31670, At1g31710 and At4g12290) are plastidial (A score of 5 was allocated to these three CuAOs using WoLF PSORT online software). Arabidopsis ADC1 (At2g16500), ADC2 (At4g34710), AIH (At5g08170) and CPAH (At2g27450) have been extensively characterized43,44,45. Abbreviations: ABAL, 4-aminobutanal; ADC, arginine decarboxylase; ALDH, aldehyde dehydrogenase; Agm, agmatine; AIH, agmatine iminohydrolase; Arg, arginine; Carbamoyl-Put, N-carbamoylputrescine; CPAH, N-carbamoylputrescine amidohydrolase; CuAO, copper amine oxidase; GABA, 4-aminobutrate; GAD, glutamate decarboxylase; PAO, polyamine oxidase; Put, putrescine; Spd, spermidine; Spm, spermine.