Figure 4

Electron Spray Ionisation-Mass Spectrometry (ESI-MS) analysis.
Mass spectrum and chemical structures: (A) UDP-GlcNAc enolpyruvate, product of MurA. (B) UDP-MurNAc, Product of MurB. (C) UDP-MurNAc–L-Alanine, product of MurC. (D) UDP-MurNAc–L-Alanine–D-Glutamate, product of MurD. (E) UDP-MurNAc–L-Alanine–D-Glutamate–meso-Diaminopimelic acid, product of MurE. (F) UDP-MurNAc pentapeptide, product of MurF. The X-axis represents the mass to charge (m/z) ratio and the Y-axis represents the relative intensity of the peaks. The data was analyzed using mMASS52, an Open Source Mass Spectrometry tool to identify the products of the various assays.