Figure 6
Optimization of the one-pot assay.
(A) Effect of ATP concentration on the one-pot assay containing all the optimized constituents after 30 mins of incubation. The X-axis represents the concentration of ATP (0.25–3 mM) and the Y-axis represents net Pi per minute (nmol Pi/min). (B) Activity of one-pot assay at different incubation periods. In B1, the X-axis represents incubation period (minutes) and the Y-axis represents net Pi per minute (nmol Pi/min) and in B2, the X-axis represents incubation period (minutes) and Y-axis represents net Pi (nmol Pi/ml). The data depicted is mean ± S.E. values obtained from three independent experiments.
