Figure 3 | Scientific Reports

Figure 3

From: Identification, Distribution and Population Dynamics of Francisella-like Endosymbiont in Haemaphysalis doenitzi (Acari: Ixodidae)

Figure 3

The vertical transmission detection of FLEs-Hd at different developmental stages of H. doenitzi.

Lanes 1 to 7 (A) Mk, DNA Marker; F, females; M, males; E, eggs; L, larvae; N, nymphs; Nc, negative control. Detection of infection sites of FLEs-Hd in different tissues of H. doenitzi. Lanes 1 to 6 (B) Mk, DNA Marker; Nc, negative control; O, ovaries; Mt, malpighian tubules; Mg, midguts; Sg, salivary glands. The horizontal transmission detection of FLEs-Hd in host animal and H. longicornis collected from the same region as H. doenitzi. Lanes 1 to 6 (C) Mk, DNA Marker; Hd, H. doenitzi; B1, blood of host animal before tick ingested; B2, blood of host animal after tick ingested; Hl, H. longicornis; Nc, negative control. The gels were run under the same experimental conditions. Cropped gels are presented (Full-length gels are shown in Supplementary Fig. 3).

Back to article page