Table 1 Oligonucleotide primers used for PCR amplification and sequencing.
Primer name | Genera or Species | Target gene | Nucleotide sequence(5′–3′) | Annealing temperature (°C) | Approx products size (bp) | Reference |
|---|---|---|---|---|---|---|
Eub 27F | Bacteria | 16S rRNA | AGAGTTTGATCCTGGCTCAG | 55 | 1500 | |
Eub 1492R | TACCTTGTTACGACTT | |||||
153F | Francisella | 16S rRNA | GCCCATTTGAGGGGGATACC | 56 | 1200 | |
1281R | GGACTAAGAGTACCTTTTTGAGT | |||||
Fran 16SqPCR F | Francisella | 16S rRNA | CAGGACTAGCTTATAGTTGCTG | 56 | 150 | This study |
Fran 16SqPCR R | CATCTGCGACAGCCTAAAAGC | |||||
Actin qPCRF | Haemaphysalis | actin | CCAGACGGAGTACTTGCGC | 55 | 100 | |
Actin qPCRR | Haemaphysalis | actin | CATGTACCCGGGCATCGCCGA |
