Figure 3
From: Nitric oxide participates in plant flowering repression by ascorbate
The activities of NO biosynthetic enzymes and the levels of NO in Oncidium PIB tissues at different stages.
(a) The NO intensity was detected using the green fluorescence of DAF-2T, and white light indicated the morphology and cell status in PIB tissues(scale bar = 50μM) (b–d) The nitric oxide (NO) levels (b), nitrate level (c) and nitrate reductase (NaR) enzymatic activity (d) were quantified in Oncidium PIB tissues at the vegetative (V), bolting (B) and reproductive (R) stages. (e) Photographs of Oncidium orchids treated with water (mock), 20 μM SNP or 100 μM SNP, which was applied on the juvenile inflorescent buds for one month. White arrows indicate the developing inflorescence (scale bar = 1cm). (f–i) Expression levels of floral genes in the inflorescence of mock or SNP-treated Oncidium orchids. The relative gene expression levels in SNP-treated plants were compared to those of mock plants. Error bars indicate standard deviation (s.d.). Asterisks represent significant differences compared with vegetative stage or mock respectively and were analyzed by two-way ANOVA (*p < 0.05; **p < 0.005; ***p < 0.0005).
