Figure 4
From: Nitric oxide participates in plant flowering repression by ascorbate
NO-related metabolism in Oncidium protocorm-like body (PLB) tissues treated with ascorbate (AsA).
(a) The NO intensity of Oncidium PLB tissues grown in ½ MS without treatment (mock), or under treatment with 1 mM and 5 mM AsA for 5 days. NO intensity was indicated by a green fluorescence of DAF-2T, and white light indicated the morphology and cell status in tissues (scale bar = 50μm). (b) The relative NO intensity in AsA-treated Oncidium PLB tissues was compared to that in mock Oncidium PLB cultures. (c,d) The relative activities of nitrate reductase (NaR) and OgNaR gene expression in AsA-treated Oncidium PLB, comparing to those of mock Oncidium. (e,f) Nitrate and arginine amounts in mock or AsA-treated Oncidium PLB tissues are shown. Significant differences compared with mock were analyzed by two-way ANOVA (*p < 0.05; **p < 0.005; ***p < 0.0005).
