Figure 1 | Scientific Reports

Figure 1

From: Purification and characterization of a novel phloretin-2′-O-glycosyltransferase favoring phloridzin biosynthesis

Figure 1

Purification steps of recombinant MdP2′GT.

(A) (a) DEAE ion-exchange chromatography fractions and (b) their corresponding specific activity. (B) (a) SDS/PAGE analysis of Ni2+ affinity chromatography fractions (FA: mixture of F2-F4 from (A); FT: FA flow-through; E1-E11: fractions eluted by buffer C containing 5 mM, 10 mM, 20 mM, 40 mM, 60 mM, 80 mM, 100 mM, 200 mM, 300 mM, 400 mM, and 500 mM imidazole, respectively) and (b) their corresponding specific activity. (C) Further purified of E6 in (B) by G75 Superdex gel filtration (a(1)elution fractions G1-G3 from E6, a(2)elution fraction G3 purified by gel filtration again) and (b) the corresponding specific activity. (D) (1) SDS/PAGE and (2) Western Blot analysis of purified MdP2′GT, M: SuperSignal Molecular Weight Protein Ladder.

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