Table 4 Enzyme characters and kinetic parameters of purified MdP2′GT.
Donor/acceptor combinations | Acceptor class | Products | Donors | Acceptors | Specific activity (μkat/Kg protein) | pH optimum | Temperature optimum (°C) | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Km (μM) | kcat (s−1) | kcat/Km (s−1. μM−1) | Km (μM) | kcat (s−1) | kcat/Km (s−1. μM−1) | ||||||
UDPG/Phloretin | Dihydrochalcone | Phloridzin | 7.53 ± 1.35 | 21.33 ± 0.68 | 2.83 | 0.50 ± 0.02 | 9.87 ± 0.89 | 19.73 | 46.82 ± 3.45 | 8.0 | 30 |
ADPG/Phloretin | Dihydrochalcone | Phloridzin | 58.67 ± 5.63 | 4.65 ± 0.14 | 0.08 | 4.09 ± 0.14 | 2.95 ± 0.10 | 0.72 | 4.90 ± 0.98 | 8.0 | 30 |
UDPG/Isoliquiritigenin | Chalcone | Isoliquiritin | 36.19 ± 4.09 | 8.68 ± 0.26 | 0.24 | 2.40 ± 0.08 | 4.35 ± 0.15 | 1.81 | 10.37 ± 1.56 | 7.5 | 40 |
UDPG/Kaempferol | Flavonol | Astragaline | 30.43 ± 3.11 | 10.83 ± 0.47 | 0.36 | 2.09 ± 0.12 | 5.65 ± 0.65 | 2.70 | 17.13 ± 1.43 | 8.5 | 35 |
ADPG/Quercetin | Flavonol | Isoquercitrin | 14.57 ± 2.45 | 15.22 ± 0.56 | 1.04 | 1.06 ± 0.08 | 7.41 ± 0.70 | 6.99 | 32.18 ± 2.95 | 8.5 | 35 |
UDP-Gal/Quercetin | Flavonol | Hyperoside | 26.19 ± 3.23 | 13.18 ± 0.52 | 0.50 | 1.81 ± 0.10 | 6.18 ± 0.23 | 5.24 | 23.13 ± 2.03 | 8.0 | 35 |
UDPG/Luteolin | Flavone | Cynaroside | 42.59 ± 4.81 | 5.95 ± 0.09 | 0.14 | 2.89 ± 0.18 | 3.24 ± 0.18 | 1.12 | 7.80 ± 1.13 | 7.5 | 40 |
