Table 5 Enzyme characters and Kinetic parameters of MdP2′GT and reported P2′GTs.

From: Purification and characterization of a novel phloretin-2′-O-glycosyltransferase favoring phloridzin biosynthesis

Enzyme

a Km (μM)

a kcat (s−1)

a kcat/Km (s−1. μM−1)

a Vmax (mmol/s. kg protein)

a Optimal pH

a Optimal temperature (°C)

Substrate specificity (Donor/acceptor combinations)

Organisms

References

MdP2′GT

0.50

9.87

19.74

167.21

8.0

30

UDPG/Phloretin, ADPG/Phloretin, UDPG/Isoliquiritigenin, UDPG/Kaempferol, ADPG/Quercetin, UDP-Gal/ Quercetin, and UDPG/Luteolin

M. x domestic a cv. Golden delicious

This study

UGT88F1

0.62

9.72c

15.68

164.75b

7.7

25

UDPG/Phloretin, UDP-galactose/ Phloretin

M. x domestica cv. Royal Gala

19

UGT88F2

—

—

—

—

8.0

45

UDPG/Phloretin, UDP/Phloridzin

P. communis cv. Abbé Fetel

7, 8, 20

UGT71A15

82

0.42d

0.005

8 × 10−3

6.75

30

UDPG/Phloretin, UDPG/Naringenin chalcone, UDPG/Eriodictyol chalcone, UDPG/Isoliquiritigenin, UDPG/Butein, UDPG/Naringenin, UDPG/Eriodictyol, UDPG/Luteolin, UDPG/Kaempferol, and UDPG/Quercetin

M. x domestica cv. Rebella

20, 21

UGT71K1

—

—

—

—

6.75

40

UDPG/Phloretin, UDPG/Kaempferol, UDPG/Quercetin, UDPG/Isoliquiritigenin, UDPG/Butein

M. x domestica cv. Rebella

20

UGT71K2

—

—

—

—

6.25

25

UDPG/Phloretin, UDPG/Kaempferol, UDPG/Quercetin, UDPG/Isoliquiritigenin, UDPG/Butein

P. communis cv. Abbé Fetel

20

UGT71A16

—

—

—

—

6.75

30

UDPG/Phloretin, UDPG/Eriodictyol, UDPG/Apigenin, UDPG/Luteolin, and UDPG/Caffeic acid

P. communis cv. Abbé Fetel

20

eYjiC

—

—

—

—

8.0

30

UDPG/Phloretin, UDPG/geldanamycin analogs

Bacillus licheniformis

3, 38

DicGT4

—

—

—

—

6.0

30

UDPG/Phloretin, UDPG/Naringenin, UDPG/Kaempferol, UDPG/Quercetin, UDPG/apigenin, and UDPG/cyanidin

Dianthus caryophyllus

14

  1. aData was determined for the glycosylation of 2′-OH group on phloretin using the UDPG/Phloretin combination as substrate.
  2. bCalculated according to the turnover rate of 9.72 × 10−4 mol.s−1.mol−1 and MW = 59 kDa.
  3. cCalculated according to Km and MW = 59 kDa.
  4. dCalculated according to Km and MW = 52 kDa.
  5. eGeldanamycin analogs act as the acceptors of YjiC included 18-dehydroxy-17-demethoxyreblastatin, 18-dehydroxy-17-O-demethylreblastatin, 18-dehydroxy-17-O-demethyl-4,5-dehydroreblastatin, 17-demethoxy-reblastatin, and 7-demethoxy-15-hydroxylreblastatin.
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