Figure 3
PTP1B affects PITX-1/RasGAP in CRC.
(A) Overexpression of PTP1B resulted in downregulation of PITX1 and p120RasGAP. DLD1 and Hct-116 cells with and without PTP1B ectopic overexpression were harvested and analyzed by western blot. Representative image of western blot were shown (N = 3). (B) Siliencing of PTP1B leaded to augmentation of PITX1 and p120RasGAP expression in CRC cells. DLD1 and Hct-116 cells with and without knockdown of PTP1B were harvested and analyzed by western blot. Representative image of western blot were shown (N = 3). (C) Expression of PITX1 and p120RasGAP were highly correlated in CRC cells. Expression of PITX1 and p120RasGAP in CRC cells were examined and quantified. Represensative western blot images were shown in upper panel (N = 3). The correlation of protein expression was determined by linear regression (lower panel).
