Figure 2
From: Identification of Comamonas testosteroni as an androgen degrader in sewage
UPLC-APCI-MS/MS analysis of the ethyl acetate extracts of the DHSTP sewage treatment samples.
(A) Total ion chromatograms of the active sewage incubated without testosterone. (B) Total ion chromatograms of the autoclaved sewage incubated with testosterone. (C) The active sewage incubated with testosterone. (CI) Total ion chromatograms of the androgen metabolites. (CII) Extracted ion chromatograms for 2, 3-SAOA (m/z = 305.21; expected retention time at 4.87 min) in the testosterone-treated sewage. (CIII) Extracted ion chromatograms for 3-HSA (m/z = 301.18) in the testosterone-treated sewage. (CIV) The MS/MS fragmentation spectra of the authentic standard (top) and the 3-HSA extracted from the testosterone-treated sewage (bottom). Abbreviations: ADD, androsta-1, 4-diene-3, 17-dione; dT, 1-dehydrotestosterone; 3-HSA, 3-hydroxy-9, 10-seco-androsta-1, 3, 5(10)-triene-9, 17-dione; 2, 3-SAOA, 17-hydroxy-1-oxo-2, 3-seco-androstan-3-oic acid; T, testosterone.
