Figure 1 | Scientific Reports

Figure 1

From: Hypoxia-inducible factor-2 alpha promotes the proliferation of human placenta-derived mesenchymal stem cells through the MAPK/ERK signaling pathway

Figure 1

Comparisons of the four types of processed hPMSCs to explore the functions of HIF-2α.

(A) (I): Morphologies of the four groups of processed hPMSCs using confocal microscopy (20×, scale bars, 50 μm). (A) (II): GFP expression in the four groups of processed hPMSCs, as assessed by the FACS assay. (a) hPMSCs-empty vector-NC, (b) hPMSCs over-expressing HIF-2α, (c) hPMSCs-scramble shRNA-NC, and (d) HIF-2α-silenced hPMSCs. (B) Analysis of the surface antigens (CD73, CD90, CD105, CD34, and CD45) on hPMSCs from the different treatment conditions: (I) untreated control hPMSCs, (II) hPMSCs-empty vector-NC, (III) hPMSCs over-expressing HIF-2α, (IV) hPMSCs-scramble shRNA-NC, and (V) HIF-2α-silenced hPMSCs. (C) Statistical graph of the comparisons of the cell proliferation rates of the different processed hPMSCs determined using EdU flow cytometry. The tests were performed in triplicate and repeated in three independent experiments. The data are presented as the mean ± S.D. (error bars) and were statistically analyzed using one-way ANOVA. **p < 0.01, ***p < 0.001.

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