Figure 2 | Scientific Reports

Figure 2

From: Hypoxia-inducible factor-2 alpha promotes the proliferation of human placenta-derived mesenchymal stem cells through the MAPK/ERK signaling pathway

Figure 2

Relative mRNA expression levels of specific genes in the different groups of processed hPMSCs.

(A) Quantitative real-time polymerase chain reaction (qRT-PCR) results from hPMSCs over-expressing HIF-2α compared with the hPMSCs-empty vector-NC. The tests were performed in triplicate and repeated in three independent experiments. The data are presented as the mean ± S.D. (error bars) and were statistically analyzed using Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001. (B,C) The qRT-PCR results from the HIF-2α-silenced hPMSCs (B: shRNAs 1545, C: shRNAs 417) compared with the hPMSCs-scramble shRNA-NC. The tests were performed in triplicate and repeated in three independent experiments. The data are presented as the mean ± S.D. (error bars) and were statistically analyzed using Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001.

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