Table 1 Sequences of ligation adapters, pre-amplification primers and selective amplification primers.
Ligation adapters | |
EcoRI-F | 5′-CTCGTAGACTGCGTACC-3′ |
EcoRI-R | 5′-AATTGGTACGCAGTCTAC-3′ |
HpaII-MspI-F | 5′-GACGATGAGTCTAGAA-3′ |
HpaII-MspI-R | 5′-CGTTCTAGACTCATC-3′ |
Pre-amplification primers | |
EcoRI (+0) | 5′-GACTGCGTACCAATTC-3′ |
HpaII-MspI (+A) | 5′-GATGAGTCTAGAACGGA-3′ |
Selective amplification primers | |
Eco-AA | 5′-GACTGCGTACCAATTCAA-3′ |
Eco-AT | 5′-GACTGCGTACCAATTCAT-3′ |
Eco-AG | 5′-GACTGCGTACCAATTCAG-3′ |
Eco-AC | 5′-GACTGCGTACCAATTCAC-3′ |
HpaII-MspI-AAT | 5′-GATGAGTCTAGAACGGAAT-3′ |
HpaII-MspI-ACT | 5′-GATGAGTCTAGAACGGACT-3′ |
HpaII-MspI-ATC | 5′-GATGAGTCTAGAACGGATC-3′ |
