Table 2 Proportion of methylation-sensitive restriction band patterns found in the CNS of locusts of different phase state, and their corresponding methylation status; methylated cytosines are indicated in bold type.

From: Acute and chronic gregarisation are associated with distinct DNA methylation fingerprints in desert locusts

Banding patterna

Methylation

solitary

24 h crowded

crowd-reared

HPA+/MSP+

noneb 5′−CCGG  GGCC−5′

11.8%

15.0%

16.3%

HPA+/MSP−

hemib 5′−CCGG GGCC−5′

10.1%

11.6%

13.4%

HPA−/MSP+

full internal 5′−CCGG GGCC−5′

12.5%

12.2%

14.4%

HPA−/MSP−

hyperc 5′-CCGG GGCC-5′

65.6%

61.2%

55.9%

  1. a+ and − indicate the presence and absence, respectively, of a band following digestion with HpaII or MspI.
  2. bMay indicate methylation of either outer or both cytosines on one strand.
  3. cHPA−/MSP− was taken to indicate hypermethylation rather than absence of target due to a genetic mutation29.
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