Figure 1 | Scientific Reports

Figure 1

From: Shedding light on cell compartmentation in the candidate phylum Poribacteria by high resolution visualisation and transcriptional profiling

Figure 1

Identification of Poribacteria cells in the sponge microbiome using FISH-CLEM.

(A) Scanning electron microscopy images (SEM) were correlated with fluorescence signal of the nucleotide stain DAPI (blue; CLEM) and the Poribacteria specific 16S rRNA probe POR1130 (red; Alexa546; FISH-CLEM) allowing the identification of microbes within the host in close proximity to a sponge cell at ultrastructure resolution. Separate channels are shown in Figure S1. (B) Three-dimensional reconstruction of a representative Poribacteria cell. Polar spherical structures (blue), which represent about 2% of cell volume, are typically observed at different z-intervals. Cell envelope is shown in green. Right panel displays FISH-CLEM micrographs used as basis for the reconstruction, where consistent POR1130 signals were observed across 10 consecutive slices totaling 1 μm of depth in z-dimension (z-values in nm). Scale bars, 2 μm (A) and 500 nm (B).

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