Figure 7 | Scientific Reports

Figure 7

From: β-Synuclein suppresses both the initiation and amplification steps of α-synuclein aggregation via competitive binding to surfaces

Figure 7

The interaction between β-synuclein monomers and α-synuclein fibrils at pH 4.8.

(a) Fluorescence microscopy of monomeric Alexa-568-β-synuclein in microcapillaries in the presence of unlabeled α-synuclein fibrils at pH 6.5 (top) and pH 4.8 (centre). The bottom capillary shows Alexa-568-β-synuclein at pH 4.8 in the absence of α-synuclein fibrils. The image in the centre illustrates the binding of β-synuclein to α-synuclein fibrils at pH 4.8, as well as the higher order assembly of the fibrils. (b) Quartz crystal microbalance (QCM) measurements at pH 4.8. α-synuclein monomers (green) or fibrils (red, blue) were covalently attached to the surface of the QCM sensors. Each trace corresponds to the change in frequency of the corresponding QCM sensor upon incubation with β-synuclein monomers as indicated in the figure.

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