Figure 6

NA assay and plaque reduction assay.
(a) NA assay. Influenza A/WSN/33 was incubated with TDSWex or zanamivir for 30 min before addition of MU-NANA (60 μM) and then incubated at 37 °C for 1 h. The controls were mock mixed with DMSO (0.3%) or virus mixed with DMSO (0.3%). The results are presented as ratios of relative NA activity ± SD from three independent experiments. (b) Plaque reduction assay. MDCK cells were infected with influenza A/WSN/33 and the infected cells were then maintained and overlaid with 0.3% agarose containing the indicated concentrations of TDSWex. The number of plaques was calculated on the right. The data are presented as means ± SD of results from three independent experiments. ***P < 0.001.